|Application ||WB, IP|
|Application & Usage||Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP can be used as positive controls.|
|Other Names||Green Fluorescent Protein|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-GFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||GFP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
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