|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||23662 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). Based on researcher’s feedback, the antibody also works in immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes GDNF of human, mouse, and rat origins. Jurkat cell lysate can be used as a positive control.|
|Other Names||neurotrophic factor GDNF, GDNF, Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor; ATF|
|Formulation||100 µg (0.2 mg/ml) protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||GDNF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.|
|Tissue Location||Expressed in both the central nervous system (CNS) and in non-CNS tissues. Expressed in a highly dynamic pattern in the anterior neuroectoderm during the early stages of neurogenesis between E7.5 and E10.5. Beginning at E10.5, expression begins in mesenchymal tissues of several organs including the digestive tract, kidney, testis, frontonasal mass, tooth primordium, tongue, mandible, whisker follicles, ear, eye, limb bud and in distinct regions of the brain. Also expressed in the heart, ileum, liver and muscle.|
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Glial cell line-derived neurotrophic factor (GDNF) has been identified as a potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons. GDNF is a glycosylated, disulfide-bonded homodimer. It promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. On the basis of these findings, it has been s µggested that GDNF may play a role in the treatment of Parkinson’s disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
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