|Application ||WB, E|
|Calculated MW||18045 Da|
|Positive Control||ELISA: Recombinant hIL-17F|
|Application & Usage||1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-17F is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
2) ELISA: Use 0.5 - 2.0 µg/ml (100 µl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17F (50.0 ng/ml), a concentration of 0.9-1.2 µg/ml of this antibody is required.
|Other Names||IL17A/F, IL17 A/F, IL-17A/F, IL-17 A/F, IL17AF, IL-17, AF, Interleukin-17 A/F, Interleukin-17 AF|
|Formulation||A sterile filtered antibody solution in PBS, pH 7.2.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IL-17F Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation (By similarity).|
|Tissue Location||Expressed in activated, but not resting, CD4+ T-cells and activated monocytes|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
IL-17F, a member of the IL-17 family of structurally related cytokines, has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis.
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