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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   sRANK Ligand Antibody   

sRANK Ligand Antibody

Rabbit Polyclonal Antibody

     
  • E - sRANK Ligand Antibody ABV10836
    To detect msRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant msRANKL.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O35235
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35003 Da
Additional Information
Gene ID 21943
Positive Control ELISA: Recombinant msRANKL
Application & Usage 1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant murine sRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 µg/ml (100 µl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of msRANKL (50.0 ng/ml), a concentration of 0.05 µg/ml of this antibody is required.
Other Names soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor)
Target/Specificity sRANKL
Antibody Form Liquid
Appearance Liquid
Formulation A sterile filtered antibody solution in PBS, pH 7.2.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionssRANK Ligand Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tnfsf11
Synonyms Opgl, Rankl, Trance
Function Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:24039232). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).
Cellular Location Isoform 1: Cell membrane; Single-pass type II membrane protein Isoform 3: Cytoplasm.
Tissue Location Highly expressed in thymus and lymph nodes, but not in non-lymphoid tissues and is abundantly expressed in T- cells but not in B-cells. A high level expression is also seen in the trabecular bone and lung
Research Areas
Citations (0)

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Background

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.

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$ 240.00
Cat# ABV10836
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