IL-3 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P08700 |
Other Accession | AAA59146 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 17233 Da |
Gene ID | 3562 |
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Application & Usage | Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human IL-3 can be used as a positive control |
Other Names | IL-3, IL3, IL 3, Interluekin-3, Interluekin 3, interluekin |
Target/Specificity | IL-3 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-IL-3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | IL-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | IL3 (HGNC:6011) |
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Function | Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage- restricted cells (PubMed:2556442). Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated (PubMed:10779277, PubMed:32889153). In addition, plays an important role in neural cell proliferation and survival (PubMed:23226269). Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed:12816992). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (PubMed:29374162). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non- hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (PubMed:27862234). |
Cellular Location | Secreted. |
Tissue Location | Activated T-cells, mast cells, natural killer cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
IL-3 is a species-specific colony-stimulating factor that stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Recombinant human IL-3 is a 15.0 kDa protein containing 133 amino acid residues.
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