|Application ||WB, E|
|Calculated MW||11098 Da|
|Application & Usage||Western blot analysis (0.5-1 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||IL-8, IL8, IL 8, Interluekin-8, Interluekin 8, interluekin|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-human IL-8 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IL-8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.|
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Provided below are standard protocols that you may find useful for product applications.
IL-8 is a proinflammatory CXC chemokine that can signal thro µgh the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils.
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