|Calculated MW||35478 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human sRANK Ligand can be used as a positive control.|
|Other Names||RANK Ligand, RANKLigand, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, Osteoprotegerin ligand|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-hsRANKL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||RANKL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||OPGL, RANKL, TRANCE|
|Function||Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type II membrane protein Isoform 2: Cytoplasm.|
|Tissue Location||Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid|
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Provided below are standard protocols that you may find useful for product applications.
Human RANK (receptor activator of NF-kB) is a member of the TNFR family, identified as a dentritic cell membrane protein. Human soluble RANK Ligand is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues).
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