|Application ||WB, IHC|
|Calculated MW||25283 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Recombinant murine VEGF can be used as a positive control.|
|Other Names||vascular endothelial growth factor|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-mouse VEGF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||VEGFAntibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity).|
|Cellular Location||Isoform VEGF-1: Secreted. Isoform VEGF-3: Cell membrane; Peripheral membrane protein. Note=Remains cell-surface associated unless released by heparin|
|Tissue Location||In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults|
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Provided below are standard protocols that you may find useful for product applications.
VEGF (Vascular Endothelial Growth Factor) is a secreted homodimeric protein, secreted by a variety of vascularized tissues. VEGF stimulates endothelial cell growth, angiogenesis, and capillary permeability.
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