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Anti-Osteopontin (NT) (Clone 2F10)

Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10451
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1κ
Clone Names 2F10
Calculated MW 35423 Da
Additional Information
Gene ID 6696
Positive Control Native Human OPN
Other Names OPN, BNSP, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin.
Target/Specificity Osteopontin
Antibody Form Liquid
Appearance Colorless liquid
Formulation 1 mg/ml in 0.15 M PBS
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsAnti-Osteopontin (NT) (Clone 2F10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPP1
Synonyms BNSP, OPN
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Cellular Location Secreted.
Tissue Location Bone. Found in plasma.
Research Areas
Citations (0)

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Human Osteopontin (OPN) is a negatively charged hydrophilic protein of 314 amino acids and is subject to significant post translational modifications (PTM) including phosphorylation and glycosylation. Due to its acidic nature and PTMs, OPN runs anomalously by SDS-PAGE. Altho µgh its mass is 35kD, apparent molecular weights may range up to 75kD. In addition, OPN is subject to proteolytic modification into smaller molecular weight fragments. The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been s µggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers. The newly exposed SVVYG epitope on the N-terminal fragment has also been shown to participate in cell adhesion.

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$ 265.00
Cat# ABV10969
(40 western blots)
Availability: 5-7days
Bulk Size
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