|Calculated MW||12367 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||DAP-12, DAP 12, DAP12, TYRO protein tyrosine kinase binding protein , TYROBP|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-DAP12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DAP12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
DAP12 (KARAP) is a natural killer (NK) cell-expressed cell surface receptor and belongs to the immunoglobulin and C-type lectin superfamily. Unlike other members of this family which possess immunoreceptor tyrosine-based inhibitory motifs (ITIM), DAP12 contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and is present as a disulphide-bonded homodimer. Crosslinking of DAP 12-KIR complexes (membrane glycoproteins of the killer-cell inhibitory receptor family without an ITIM in their cytoplasmic domain) results in cellular activation.
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