|Application ||WB, IHC, IP|
|Calculated MW||41758 Da|
|Positive Control||Recombinant humanHDAC8|
|Application & Usage||Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually.|
|Other Names||Histone deacetylase 8|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-HDAC8 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HDAC8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.|
|Cellular Location||Nucleus. Cytoplasm. Note=Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation|
|Tissue Location||Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney|
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Provided below are standard protocols that you may find useful for product applications.
Mammalian HDAC-8, isolated from human kidney, is a member of the histone deacetylase family. HDAC-8 shares homology with other HDACs in the family, but has different tissue distribution. HDAC-8 is localized to the nucleus and plays a role in the tissue development as well as cancer etiology.
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