|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||29266 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). Based on researcher’s feedback, it can also be used in immunoprecipitation (10-20 µg/ml); and Immunohistochemistry (15-25 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes TCR α in human sample, and to a lesser extent to mouse and rat samples. Jurkat cell lysate can be used as a positive control.|
|Other Names||T cell antigen receptor, T cell receptor alpha, TCRA, TCRD|
|Formulation||100 µg (0.2 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TCR alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow|
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Provided below are standard protocols that you may find useful for product applications.
TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either α and β or γ and δ chains. The vast majority of circulating T cells (95%) express the α/β heterodimer while ro µghly 2-5% express the γ/δ heterodimer. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction thro µgh the TCR.
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