|Positive Control||Recombinant human IGF-BP1|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human IGFBP-1 can be used as a positive control. Other applications have not been determined.|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-human IGF-BP1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IGF-BP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Human IGF-BP1 is a cysteine-rich secreted protein expressed in liver, decidual, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory.
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