|Calculated MW||14284 Da|
|Application & Usage||Western blot analysis (0.5-2 µg/ml). Per researchers feedback, it can also be used in ELISA (2-3 µg/ml) and neutralization (3-6 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||TFF-2, TFF 2, TFF2, trefoil factor 2, Spasmolytic polypeptide, SP, Spasmolysin|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-human TFF-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TFF-2/Spasmolysin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data s µggests a potential role for TFF2 in acute and chronic asthma. Human TFF2 is a 12.0 kDa polypeptide of 106 amino acid residues.
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