|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant CFP can be used a positive control.|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-CFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||CFP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. The protein is suitable as a control reagent for CFP expression studies or as a labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis of CFP transfected cells, label other proteins, calibration of fluorometers and flow cytometers, fluorescence microscope, or microinjection of CFP into cells and tissues, etc.
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