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>   home   >   Products   >   Primary Antibodies   >   MBD2/3 (Clone 106B691) Antibody   

MBD2/3 (Clone 106B691) Antibody

Mouse Monoclonal Antibody

  • WB - MBD2/3 (Clone 106B691) Antibody ABV11102
    Lane1:Hela cell nuclear fraction(Absence of Immunizing peptide; Lane2:Hela cellnuclear fraction(Presence of Immunizing peptide)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95983
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1κ
Clone Names 106B691
Calculated MW 32844 Da
Additional Information
Gene ID 53615
Positive Control HeLa cell nuclear fraction
Application & Usage Western blot analysis (2-4 µg/ml). However, the optimal conditions should be determined individually.
Other Names Methyl-CpG-Binding Domain 2/3
Target/Specificity MBD2/3
Antibody Form Liquid
Appearance Colorless liquid
Formulation 50 µg in 100 µl PBS containing 0.2% gelatin and 0.05% sodium azide
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsMBD2/3 (Clone 106B691) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MBD3
Function Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
Cellular Location Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes
Citations (0)

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DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. A demethylase enzyme has been identified which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) co-localize with foci of heavily methylated satellite DNA and mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA either in vivo or in vitro. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors, MBD4 is mutated at an exonic polynucleotide tract.

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$ 225.00
Cat# ABV11102
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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