|Reactivity||Human, Mouse, Rat|
|Calculated MW||58897 Da|
|Positive Control||Western Blot: Various cell lysates|
|Application & Usage||Western blot: 1:500 – 1:1000 dilution. However, the optimal conditions should be determined individually.|
|Other Names||USP3, MGC129878, MGC129879, SIH003, UBP|
|Formulation||100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||USP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.|
|Cellular Location||Nucleus. Note=Localizes preferentially with monoubiquitinated H2A to chromatin|
|Tissue Location||Expressed in all tissues examined, with strongest expression in pancreas|
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Provided below are standard protocols that you may find useful for product applications.
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP3 (ubiquitin specific peptidase 3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBPtype zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin Cterminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
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