|Application ||WB, ICC, E, IP|
|Isotype||Mouse IgG 1|
|Calculated MW||22086 Da|
|Positive Control||IP analysis : HeLa cell lysate. IHC staining : HeLa cells|
|Application & Usage||Western blot: 1:1000, IP: 1-2 µl, ICC: 20 µl/ml, ELISA.|
|Other Names||Peroxiredoxin 5, ACR1, AOEB116, B116, PLP, PMP20, PRDX6, PRXV, SBBI10.|
|Formulation||100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Peroxiredoxin V Antibody (3F11) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.|
|Cellular Location||Isoform Mitochondrial: Mitochondrion.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx V is involved in reduction of hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
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