|Application ||WB, E|
|Reactivity||Human, Mouse, Rat|
|Isotype||Mouse IgG 1|
|Calculated MW||25035 Da|
|Positive Control||WB analysis: HeLa, Mouse lung, Rat lung lysates. IHC staining: paraffin embedded human kidney|
|Application & Usage||Western blot: 1:2000, ELISA.|
|Other Names||Peroxiredoxin 6, 1-Cys, aiPLA2, AOP2, NSGPx, p29, PRX.|
|Formulation||100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Peroxiredoxin VI Antibody (4A3) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.|
|Cellular Location||Cytoplasm. Lysosome. Cytoplasmic vesicle. Note=Also found in lung secretory organelles.|
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Provided below are standard protocols that you may find useful for product applications.
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx VI is involved in redox regulation of the cell. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
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