|Application ||WB, E, IP|
|Isotype||Mouse IgG 1|
|Calculated MW||32251 Da|
|Positive Control||WB analysis of cell lysates.|
|Application & Usage||Western blot: 1:1000, IP: 1-2 µl, ELISA.|
|Other Names||TXN, TRX, TRX1.|
|Formulation||100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Thioredoxin 1 Antibody (3A1) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||TRP32, TXL, TXNL|
|Function||Active thioredoxin with a redox potential of about -250 mV.|
|Cellular Location||Cytoplasm. Nucleus. Note=At least 85% of the cellular TXNL1 is proteasome-associated|
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Provided below are standard protocols that you may find useful for product applications.
The mammalian thioredoxin reductases (TrxRs) are a family of seleno-cysteine containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate seleno-cysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55 – 58 kDa in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. TrxR1 plays a central role as a glucosyl donor in cellular metabolic pathways.
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