|Calculated MW||79542 Da|
|Positive Control||Western Blot: Recombinant protein|
|Application & Usage||Western blot: 1-4 µg/ml.|
|Other Names||KIAA0043, RING3L|
|Formulation||100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BRD3-BD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.|
|Cellular Location||Nucleus. Note=Detected on chromatin.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRD3 binds hyper-acetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. It regulates transcription by promoting the binding of the transcription factor GATA1 to its targets and transcription of the CCND1 gene. A chromosomal aberration involving BRD3 was found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.