|Calculated MW||79495 Da|
|Positive Control||Western Blot: Jurkat cell lysate, 3T3 cell lysate|
|Application & Usage||Western blot: 1-4 µg|
|Other Names||Enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase, LBFP, LBP, L-PBE, peroxisomal bifunctional enzyme3, 2-trans-enoyl-CoA isomerase, peroxisomal enoyl-CoA hydratase|
|Formulation||100 µg (0.5 mg/ml) of Caspase-10 antibody in PBS pH 7.2, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EHHADH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.|
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Provided below are standard protocols that you may find useful for product applications.
EHHADH is a bifunctional enzyme. It is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of this protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. EHHADH-null mice only exhibit a blunted peroxisome proliferative response when challenged with a peroxisome proliferator.
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