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>   home   >   Products   >   Primary Antibodies   >   His-Tag Antibody (Clone 11E12)   

His-Tag Antibody (Clone 11E12)

Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity All Species
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1 κ
Clone Names Clone 11E12
Additional Information
Positive Control Western blot: His-Tagged protein
Application & Usage ELISA, WB
Other Names His-Tag
Target/Specificity His-Tag
Antibody Form Liquid
Appearance Colorless liquid
Formulation 1 mg/ml in 0.15 M PBS
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsHis-Tag Antibody (Clone 11E12) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

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$ 325.00
Cat# ABV11264
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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Ordering Information

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