|Application ||WB, IHC, FC|
|Calculated MW||59756 Da|
|Positive Control||Western blot: Mouse kidney tissue lysate, IHC: human lung carcinoma, FACS: 293 cells|
|Application & Usage||Western blot: ~1:1000, IHC: ~1:50–1:100, FACS: ~1:10-1:50.|
|Other Names||CAT; Catalase|
|Formulation||100 µl of antibody in PBS with 0.09% (W/V) sodium azide|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Catalase Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.|
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Provided below are standard protocols that you may find useful for product applications.
Catalase is a peroxisome specific marker protein belonging to the catalase family. Defects in the gene encoding for the catalase protein can cause acatalasia, a disease characterized by the absence of catalase activity in red cells and associated with ulcerating oral lesions. Catalase is also an important regulator of oxidative stress and inflammation, and may contribute to the development of rheumatoid arthritis. Catalase, which can form a homotetramer, is found in all nearly all aerobically respiring organisms and functions in protecting cells from the toxic effects of hydrogen peroxide.
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