|Calculated MW||83055 Da|
|Positive Control||Western blot: HeLa cells, ChIP: Mouse ES cells.|
|Application & Usage||ChIP: 10 µg/ChIP, Western Blot: 1:1000|
|Other Names||CHET9, JJAZ1|
|Formulation||In PBS with 0.05% sodium azide and 0.05% ProClin 300.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Suz12 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CHET9, JJAZ1, KIAA0160|
|Function||Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.|
|Tissue Location||Overexpressed in breast and colon cancer.|
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Provided below are standard protocols that you may find useful for product applications.
Suz12 is a Polycomb group (PcG) protein. These proteins form multiprotein complexes, which keep homeotic genes in a transcriptionally repressive state throughout development. PcG proteins are not required to initiate repression, but maintain it during later stages of development. They probably act via the methylation of histones. Suz12 is a component of the PRC2 complex, which is composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7, and methylates ‘Lys-9’ and ‘Lys-27’ of histone H3.
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