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JMJD2b polyclonal antibody

Rabbit Polyclonal Antibody

  • E - JMJD2b polyclonal antibody ABV11386
    To determine the titer, an ELISA was performed using a serial dilution of the antibody. The wells were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,000.
  • WB - JMJD2b polyclonal antibody ABV11386
    Nuclear extracts of HeLa cells were analyzed with the antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O94953
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 121897 Da
Additional Information
Gene ID 23030
Positive Control Western blot: HeLa cells, ELISA: Peptides, IF: HeLa cells.
Application & Usage Western Blot: 1:1000, ELISA: 1:200, IF: 1:200.
Other Names JHDM3b, KDM4b
Target/Specificity JMJD2b
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsJMJD2b polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM4B
Synonyms JHDM3B, JMJD2B, KIAA0876
Function Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00537, ECO:0000269|PubMed:15927959}
Citations (0)

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JMJD2b belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2b specifically demethylates the trimethylated K9 of histone H3. It is not able to demethylate K4, K27 and K36 of histone H3, nor K20 of histone H4.

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$ 295.00
Cat# ABV11386
(40 western blots)
Availability: 5-7days
Bulk Size
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