Jarid1b polyclonal antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9UGL1 |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 175658 Da |
Gene ID | 10765 |
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Positive Control | Western blot: NIH3T3 and embryonic stem cells, ELISA: Peptides. |
Application & Usage | Western Blot: 1:500, ELISA: 1:100 – 1:200. |
Other Names | CT31, PLU1, PUT1, RBBP2H1, RBP2-like |
Target/Specificity | Jarid1b |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | In PBS with 0.05% (W/V) sodium azide. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Jarid1b polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | KDM5B |
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Synonyms | JARID1B, PLU1, RBBP2H1 |
Function | Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:24952722, PubMed:27214403, PubMed:28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed:24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity). |
Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00355, ECO:0000255|PROSITE-ProRule:PRU00537, ECO:0000269|PubMed:10336460, ECO:0000269|PubMed:12237901} |
Tissue Location | Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
JARID1b is a histone demethylase which specifically demethylates mono-, di, and trimethylated lysine 4 of histone H3. It acts as a transcriptional repressor for the tumor suppressor genes BRCA1 and HOXA5 and has been associated with breast cancer development. In contrast, in melanoma JARID1b itself may function as a tumor suppressor.
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