|Reactivity||Human, Mouse, Rat|
|Calculated MW||28147 Da|
|Positive Control||WB: HEK293T, Raw264.7, H9C2 whole cell lysates|
|Application & Usage||WB: 1:500 - 1:1000|
|Other Names||LFA3; Lymphocyte function-associated antigen 3; Ag3; Surface glycoprotein LFA-3; CD58|
|Formulation||1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||CD58 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein|
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CD58, also known as lymphocyte function-associated antigen 3 (LFA-3) is a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily. Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on antigen presenting cells and target cells enhances T cell recognition via the binding of its cognate ligand, CD2, on the T cell surface.
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