|Application ||IHC, FC, E|
|Positive Control||IHC: liver carcinoma tissue, FCM: HepG2 and L02 cell lines|
|Application & Usage||cELISA: 1:4000 – 1:15000, FCM: 1: 400, IHC: 1: 400.|
|Other Names||S- Adenosylmethionine|
|Formulation||10 mM PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide, 50% Glycerol and 10 mg/ml BSA|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||S- Adenosylmethionine Antibody (Clone # 84-19) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
S-Adenosylmethionine (SAM) is a naturally occurring compound that is found in almost every tissue and fluid in the body. It is a common co-substrate involved in methyl group transfers. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyl transferase. Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. SAM plays a role in the immune system, maintains cell membranes, and helps produce and break down brain chemicals, such as serotonin, melatonin, and dopamine. It works with vitamin B12 and folate (vitamin B9). Being deficient in either vitamin B12 or folate may reduce levels of SAM in your body.
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