|Reactivity||Human, Mouse, Rat|
|Calculated MW||84871 Da|
|Positive Control||Western blot: Human serum, Mouse muscle lysate, Rat kidney lysate, Human Apotransferrin|
|Application & Usage||Western blot: 1-4 µg|
|Other Names||Siderophilin, TRF|
|Formulation||100 µg (0.5 mg/ml) of antibody in PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin®, and 50 % glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20°C|
|Precautions||Apotransferrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
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Provided below are standard protocols that you may find useful for product applications.
Apotransferrin is the ion-free form of Transferrin. Like transferrin, apotransferrin has a physiological role in the transportation and distribution of iron among the body organs. It is also an important transport factor used in defined culture media.
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