|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||20532 Da|
|Other Names||PUMA/JFY1 , JFY-1, PUMA, BBC3, bcl-2 binding component 3|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Precautions||PUMA/bbc3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.|
|Cellular Location||Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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