|Application ||WB, IHC|
|Calculated MW||46429 Da|
|Positive Control||Western blot, IHC|
|Application & Usage||WB: 1:200-1:2000, IHC: 1:100|
|Other Names||5-hydroxytryptamine receptor 1A, 5-HT-1A, 5-HT1A, Serotonin receptor 1A, Htr1a, 5ht1a|
|Formulation||100 µg (0.5 mg/ml) of antibody in PBS, pH 7.4, 1% BSA, 0.05% NaN3.|
|Reconstitution & Storage||-20 °C|
|Precautions||5-Hydroxytryptamine Receptor 1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5- hydroxytryptamine release and in the regulation of dopamine and 5- hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Detected in hypothalamus, mesencephalon, amygdala, medulla, thalamus, septum and hippocampus|
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Provided below are standard protocols that you may find useful for product applications.
Serotonin [5-hydroxytryptamine (5-HT1)] is an important neurotransmitter in the CNS that exerts its neuronal activity via 5-HT receptors. 5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1 The 5-HT receptor families belong to the G-Protein Coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels. The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1e AND 5-HT1F) which share 41-63% overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation. The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the central nervous system, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species. Activation of 5-HT1A receptor decreases blood pressure and heart rate, involved in learning, memory, relieving anxiety and depression. 5-Hydroxytryptamine Receptor
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