|Application ||WB, IH|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||32745 Da|
|Positive Control||WB: HeLa, HuvEc, DKD, mouse brain, rat brain lysates ; IHC: human ovarian cancer tissue|
|Application & Usage||WB; 1:500 – 1:2000, IH; 1:50 – 1:200|
|Other Names||CAF1, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, CAF-1, Caf1a|
|Handling||The antibody solution should be gently mixed before use|
|Reconstitution & Storage||-20°C|
|Precautions||Anti-CNOT7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti- proliferative activity.|
|Cellular Location||Nucleus. Cytoplasm, P-body. Note=NANOS2 promotes its localization to P-body.|
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