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Functional LAG-3 Antibody, mAb (blocking)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, E, IP |
|---|---|
| Primary Accession | P18627 |
| Reactivity | Human, Monkey |
| Host | Purified From Concentrated Hybridoma Tissue Culture Supernatant. |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Gene Source | Human |
| Endotoxin | <0.001EU/ µg purified protein or <1EU/mg purified protein (LAL test; Lonza). |
| Application Note | E,Functional Application,Inhibition,ICC,IHC,IP(10 µg/ml),WB(5 µg/ml) |
| Clone Names | 11E3 |
| Calculated MW | 57449 Da |
| Gene ID | 3902 |
|---|---|
| Other Names | Lymphocyte Activation Gene-3; FDC Protein; CD223 |
| Target/Specificity | Recognizes human and monkey LAG-3. |
| Format | Liquid. In PBS containing 0.02% sodium azide. |
| Reconstitution & Storage | Stable for at least 1 year after receipt when stored at -20°C. |
| Precautions | Functional LAG-3 Antibody, mAb (blocking) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LAG3 (HGNC:6476) |
|---|---|
| Synonyms | FDC |
| Function | Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:7805750, PubMed:8647185, PubMed:20421648). Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:7805750, PubMed:8647185, PubMed:20421648). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185). |
| Cellular Location | [Lymphocyte activation gene 3 protein]: Cell membrane; Single-pass type I membrane protein |
| Tissue Location | Primarily expressed in activated T-cells and a subset of natural killer (NK) cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Lymphocyte activation gene 3 (LAG-3; CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.
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