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Functional HMGB1 Antibody, mAb (recombinant)

  • WB - Functional HMGB1 Antibody, mAb (recombinant) ADP0039
    Western blot analysis of human and rat HMGB1 using anti-HMGB1, mAb (rec.) (GIBY-1-4)
    Different amounts of cell extracts from HEK293T cells (3ug, 5ug and 30ug) either transfected with a plasmid coding for rat HMGB1 (lanes 1, 2, 3) or non-transfected (lanes 4, 5, 6), were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-HMGB1,mAb (rec.) (GIBY-1-4) (1ug /ml). Proteins were visualized by a chemiluminescence detection system.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09429
Reactivity Human, Mouse, Rat
Host Purified From HEK 293 Cell culture Supernatant.
Clonality Monoclonal
Isotype Human IgG2λ
Gene Source Human
Application Note E,WB(1:1000)
Clone Names Giby-1-4
Calculated MW 24894 Da
Description HMGB1, monoclonal antibody (recombinant) (Giby1-4) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.
Additional Information
Gene ID 3146
Other Names High Mobility Group Protein B1
Target/Specificity Recognizes human, mouse and rat HMGB1.
Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Reconstitution & Storage Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
PrecautionsFunctional HMGB1 Antibody, mAb (recombinant) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGB1
Synonyms HMG1
Function DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)

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HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-kappa and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-kappa inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also a causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis.

Anti-HMGB1, mAb (recombinant) (Giby-1-4) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

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$ 290.00
Cat# ADP0039
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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