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AFX Antibody (S197)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AE1004a | 0.1 mg 400 ul | 7-10 days | $ 325.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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AFX Antibody (S197) - Product info | |
| Application | WB, IHC
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| Primary Accession | P98177 |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1mg/ml |
| Isotype | Rabbit IgG |
| Calculated MW | 53684 Da |
AFX Antibody (S197) - Additional info | |
| Gene ID 4303 | |
| Other Names Fork head domain transcription factor AFX1; Forkhead box protein O4 | |
| Target/Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | |
| Dilution WB~~1:500~1:1000 IHC~~1:50-1:100 | |
| Format affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AFX Antibody (S197) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AFX Antibody (S197) - Protein Information | |
| Name FOXO4 | |
| Synonyms AFX, AFX1, MLLT7 | |
| Function Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity | |
| Cellular Location Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm Dephosphorylation triggers nuclear translocation Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm | |
| Tissue Location Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas | |
AFX Antibody (S197) - Application data
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Western blot analysis of extracts from 293 cells using AFX Antibody (S197) (#AE1004a, Lane 1 and 2) and Phospho-AFX-S197 Antibody (#AE1004b, Lane 3 and 4).
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Immunohistochemical analysis of paraffin-embeddedhuman lung carcinoma tissue, using AFX Antibody (S197) (#AE1004a).
AFX Antibody (S197) - Related products
AP6193a: MLLT7 Antibody (N-term)
RI12060: FOXO4 predesign siRNA
LY10589a: FOXO4 Over-expression Lysate
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BACKGROUND
This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene.
REFERENCES
PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4. Kwon IK, et al. Oncogene, 2010 Jun 10. PMID 20348951. Effects of FoxO4 overexpression on cholesterol biosynthesis, triacylglycerol accumulation, and glucose uptake. Zhu J, et al. J Lipid Res, 2010 Jun. PMID 20037138. O-GlcNAcylation enhances FOXO4 transcriptional regulation in response to stress. Ho SR, et al. FEBS Lett, 2010 Jan 4. PMID 19932102. FoxO4 inhibits NF-kappaB and protects mice against colonic injury and inflammation. Zhou W, et al. Gastroenterology, 2009 Oct. PMID 19560465. 14-3-3 protein masks the DNA binding interface of forkhead transcription factor FOXO4. Silhan J, et al. J Biol Chem, 2009 Jul 17. PMID 19416966.
