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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AFX Antibody (S197)   

AFX Antibody (S197)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - AFX  Antibody (S197) AE1004a
    Western blot analysis of extracts from 293 cells using AFX Antibody (S197) (#AE1004a, Lane 1 and 2) and Phospho-AFX-S197 Antibody (#AE1004b, Lane 3 and 4).
  • IHC - AFX  Antibody (S197) AE1004a
    Immunohistochemical analysis of paraffin-embeddedhuman lung carcinoma tissue, using AFX Antibody (S197) (#AE1004a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P98177
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Concentration 1mg/ml
Isotype Rabbit IgG
Calculated MW 53684 Da
Additional Information
Gene ID 4303
Other Names Forkhead box protein O4, Fork head domain transcription factor AFX1, FOXO4, AFX, AFX1, MLLT7
Target/Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution WB~~1:500~1:1000
Format affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAFX Antibody (S197) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXO4
Synonyms AFX, AFX1, MLLT7
Function Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Cellular Location Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm Dephosphorylation triggers nuclear translocation Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm
Tissue Location Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas
Research Areas
Citations (0)

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This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene.


PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4. Kwon IK, et al. Oncogene, 2010 Jun 10. PMID 20348951.

Effects of FoxO4 overexpression on cholesterol biosynthesis, triacylglycerol accumulation, and glucose uptake. Zhu J, et al. J Lipid Res, 2010 Jun. PMID 20037138.

O-GlcNAcylation enhances FOXO4 transcriptional regulation in response to stress. Ho SR, et al. FEBS Lett, 2010 Jan 4. PMID 19932102.

FoxO4 inhibits NF-kappaB and protects mice against colonic injury and inflammation. Zhou W, et al. Gastroenterology, 2009 Oct. PMID 19560465.

14-3-3 protein masks the DNA binding interface of forkhead transcription factor FOXO4. Silhan J, et al. J Biol Chem, 2009 Jul 17. PMID 19416966.

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$ 325.00
Cat# AE1004a
(40 western blots)
Availability: 7-10 days
Bulk Size
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