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Goat Anti-Acylglycerol kinase Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-Acylglycerol kinase Antibody AF1023a
    AF1023a (1 µg/ml) staining of Human Brain (Substantia nigra) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q53H12
Other Accession NP_060708, 55750, 69923 (mouse)
Reactivity Human
Predicted Mouse
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 47137 Da
Additional Information
Gene ID 55750
Other Names Acylglycerol kinase, mitochondrial, hAGK,,, Multiple substrate lipid kinase, HsMuLK, MuLK, Multi-substrate lipid kinase, AGK, MULK
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Acylglycerol kinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AGK
Synonyms MULK
Function Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
Cellular Location Mitochondrion membrane
Tissue Location Highly expressed in muscle, heart, kidney and brain.
Research Areas
Citations (0)

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Expression of autotaxin and acylglycerol kinase in prostate cancer: association with cancer development and progression. Nouh MA, et al. Cancer Sci, 2009 Sep. PMID 19549252.
Substrate chirality and specificity of diacylglycerol kinases and the multisubstrate lipid kinase. Epand RM, et al. Biochemistry, 2007 Dec 11. PMID 18004883.
Functions of the multifaceted family of sphingosine kinases and some close relatives. Spiegel S, et al. J Biol Chem, 2007 Jan 26. PMID 17135245.
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.
Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies. Van Overloop H, et al. J Lipid Res, 2006 Feb. PMID 16269826.

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$ 325.00
Cat# AF1023a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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