|Application ||WB, E|
|Other Accession||NP_127462, 50649|
|Calculated MW||79067 Da|
|Other Names||Rho guanine nucleotide exchange factor 4, APC-stimulated guanine nucleotide exchange factor 1, Asef, Asef1, ARHGEF4, KIAA1112|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-ARHGEF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.|
|Cellular Location||Isoform 3: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated with membrane ruffles.|
|Tissue Location||Expressed at high levels in the brain, skeletal muscle and testis and at low levels in the kidney, lung, small intestine, ovary and prostate. Expression is aberrantly enhanced in most colorectal tumors.|
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Provided below are standard protocols that you may find useful for product applications.
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. This protein is similar to rat collybistin protein. Alternative splicing of this gene generates two transcript variants which encode different isoforms. Also there is possibility for the usage of multiple polyadenylation sites for this gene.
The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation. Lyons R, et al. Leuk Res, 2010 Jan. PMID 19628279.
Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase. Kawasaki Y, et al. J Biol Chem, 2009 Aug 14. PMID 19525225.
Phosphorylation and activation of the Rac1 and Cdc42 GEF Asef in A431 cells stimulated by EGF. Itoh RE, et al. J Cell Sci, 2008 Aug 15. PMID 18653540.
Htid-1, the human homolog of the Drosophila melanogaster l(2)tid tumor suppressor, defines a novel physiological role of APC. Kurzik-Dumke U, et al. Cell Signal, 2007 Sep. PMID 17588722.
Adenomatous polyposis coli (APC) is required for normal development of skin and thymus. Kuraguchi M, et al. PLoS Genet, 2006 Sep 15. PMID 17002498.
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