|Application ||WB, E|
|Other Accession||NP_059431, 9266, 19158 (mouse), 116692 (rat)|
|Calculated MW||46546 Da|
|Other Names||Cytohesin-2, ARF exchange factor, ARF nucleotide-binding site opener, Protein ARNO, PH, SEC7 and coiled-coil domain-containing protein 2, CYTH2, ARNO, PSCD2, PSCD2L|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-ARNO / cytohesin 2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ARNO, PSCD2, PSCD2L|
|Function||Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.|
|Cellular Location||Cell membrane; Peripheral membrane protein Cytoplasm. Note=Both isoform 1 and isoform 2 are recruited to the cell membrane through their association with ARL4A, ARL4C and ARL4D. They require also interaction with phosphoinositides for targeting to the plasma membrane|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene.
Cytohesin-2/ARNO, through its interaction with focal adhesion adaptor protein paxillin, regulates preadipocyte migration via the downstream activation of Arf6. Torii T, et al. J Biol Chem, 2010 Jul 30. PMID 20525696.
Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO. Merkulova M, et al. Biochim Biophys Acta, 2010 Aug. PMID 20153292.
GRASP and IPCEF promote ARF-to-Rac signaling and cell migration by coordinating the association of ARNO/cytohesin 2 with Dock180. White DT, et al. Mol Biol Cell, 2010 Feb 15. PMID 20016009.
Identification of a guanine nucleotide exchange factor for Arf3, the yeast orthologue of mammalian Arf6. Gillingham AK, et al. PLoS One, 2007 Sep 5. PMID 17786213.
The calcium-sensing receptor changes cell shape via a beta-arrestin-1 ARNO ARF6 ELMO protein network. Bouschet T, et al. J Cell Sci, 2007 Aug 1. PMID 17623778.
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