|Application ||WB, E|
|Other Accession||NP_003558, 8412|
|Predicted||Human, Pig, Dog, Cow|
|Calculated MW||92566 Da|
|Other Names||Breast cancer anti-estrogen resistance protein 3, Novel SH2-containing protein 2, SH2 domain-containing protein 3B, BCAR3, NSP2, SH2D3B|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-BCAR3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.|
|Tissue Location||Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue|
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Provided below are standard protocols that you may find useful for product applications.
Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48.
Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
BCAR3 regulates Src/p130 Cas association, Src kinase activity, and breast cancer adhesion signaling. Schuh NR, et al. J Biol Chem, 2010 Jan 22. PMID 19940159.
Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.
AND-34/BCAR3 regulates adhesion-dependent p130Cas serine phosphorylation and breast cancer cell growth pattern. Makkinje A, et al. Cell Signal, 2009 Sep. PMID 19454314.
BCAR3 regulates EGF-induced DNA synthesis in normal human breast MCF-12A cells. Oh MJ, et al. Biochem Biophys Res Commun, 2008 Oct 24. PMID 18722344.
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