Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Goat Anti-C14orf169 / NO66 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, Pep-ELISA |
|---|---|
| Primary Accession | Q9H6W3 |
| Other Accession | NP_078920, 79697, 71952 (mouse) |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 71086 Da |
| Gene ID | 79697 |
|---|---|
| Other Names | Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66, 1.14.11.-, 1.14.11.27, 60S ribosomal protein L8 histidine hydroxylase, Histone lysine demethylase NO66, Myc-associated protein with JmjC domain, Nucleolar protein 66, hsNO66, Ribosomal oxygenase NO66, ROX, NO66, C14orf169, MAPJD |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-C14orf169 / NO66 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RIOX1 {ECO:0000303|PubMed:35210392, ECO:0000312|HGNC:HGNC:20968} |
|---|---|
| Function | Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase (PubMed:23103944). Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity). Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2) (By similarity). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). Also catalyzes demethylation of non-histone proteins, such as CGAS: demethylation of monomethylated CGAS promotes interaction between CGAS and PARP1, followed by PARP1 inactivation (By similarity). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216', thereby playing a role in ribosome biogenesis (PubMed:23103944). Participates in MYC- induced transcriptional activation (PubMed:17308053). |
| Cellular Location | Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Granular part of nucleoli (PubMed:14742713). Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli (PubMed:14742713) |
| Tissue Location | Widely expressed. Overexpressed in lung carcinomas. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
References
Regulation of the osteoblast-specific transcription factor Osterix by NO66, a Jumonji family histone demethylase. Sinha KM, et al. EMBO J, 2010 Jan 6. PMID 19927124.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Identification of Myc-associated protein with JmjC domain as a novel therapeutic target oncogene for lung cancer. Suzuki C, et al. Mol Cancer Ther, 2007 Feb. PMID 17308053.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. Eilbracht J, et al. Mol Biol Cell, 2004 Apr. PMID 14742713.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
