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Goat Anti-C14orf169 / NO66 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-C14orf169 / NO66 Antibody AF1170a
    AF1170a (0.3 µg/ml) staining of A431 cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H6W3
Other Accession NP_078920, 79697, 71952 (mouse)
Reactivity Human
Predicted Mouse, Rat
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 71086 Da
Additional Information
Gene ID 79697
Other Names Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66, 1.14.11.-,, 60S ribosomal protein L8 histidine hydroxylase, Histone lysine demethylase NO66, Myc-associated protein with JmjC domain, Nucleolar protein 66, hsNO66, Ribosomal oxygenase NO66, ROX, NO66, C14orf169, MAPJD
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-C14orf169 / NO66 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NO66
Synonyms C14orf169, MAPJD
Function Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC- induced transcriptional activation.
Cellular Location Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Granular part of nucleoli. Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli
Tissue Location Widely expressed. Overexpressed in lung carcinomas.
Research Areas
Citations (0)

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Regulation of the osteoblast-specific transcription factor Osterix by NO66, a Jumonji family histone demethylase. Sinha KM, et al. EMBO J, 2010 Jan 6. PMID 19927124.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Identification of Myc-associated protein with JmjC domain as a novel therapeutic target oncogene for lung cancer. Suzuki C, et al. Mol Cancer Ther, 2007 Feb. PMID 17308053.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. Eilbracht J, et al. Mol Biol Cell, 2004 Apr. PMID 14742713.

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$ 325.00
Cat# AF1170a
(40 western blots)
Availability: 7-10 days
Bulk Size
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