|Application ||WB, IHC, E|
|Other Accession||NP_115791, 84433, 108723 (mouse)|
|Predicted||Mouse, Dog, Cow|
|Calculated MW||133284 Da|
|Other Names||Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1, CARD11, CARMA1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-CARD11 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the costimulatory signal essential for T- cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.|
|Cellular Location||Cytoplasm. Membrane raft. Note=Colocalized with DPP4 in membrane rafts|
|Tissue Location||Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL- 60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.
New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.
NF-kappaB activation in T cells requires discrete control of IkappaB kinase alpha/beta (IKKalpha/beta) phosphorylation and IKKgamma ubiquitination by the ADAP adapter protein. Srivastava R, et al. J Biol Chem, 2010 Apr 9. PMID 20164171.
Phosphorylation of CARMA1 by HPK1 is critical for NF-kappaB activation in T cells. Brenner D, et al. Proc Natl Acad Sci U S A, 2009 Aug 25. PMID 19706536.
COP9 signalosome controls the Carma1-Bcl10-Malt1 complex upon T-cell stimulation. Welteke V, et al. EMBO Rep, 2009 Jun. PMID 19444310.
Compensatory IKKalpha activation of classical NF-kappaB signaling during IKKbeta inhibition identified by an RNA interference sensitization screen. Lam LT, et al. Proc Natl Acad Sci U S A, 2008 Dec 30. PMID 19104039.
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