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Goat Anti-CARD11 Antibody

Peptide-affinity purified goat antibody

  • IHC - Goat Anti-CARD11 Antibody AF1190a
    AF1190a (2 µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • WB - Goat Anti-CARD11 Antibody AF1190a
    AF1190a staining (1 µg/ml) of human Jurkat cells (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • WB - Goat Anti-CARD11 Antibody AF1190a
    HEK293 overexpressing CARD11 (RC222740) and probed with AF1190a (mock transfection in first lane), tested by Origene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BXL7
Other Accession NP_115791, 84433, 108723 (mouse)
Reactivity Human
Predicted Mouse, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 133284 Da
Additional Information
Gene ID 84433
Other Names Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1, CARD11, CARMA1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CARD11 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CARD11
Synonyms CARMA1
Function Involved in the costimulatory signal essential for T- cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Cellular Location Cytoplasm. Membrane raft. Note=Colocalized with DPP4 in membrane rafts
Tissue Location Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL- 60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells
Research Areas
Citations (0)

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The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.


New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.
NF-kappaB activation in T cells requires discrete control of IkappaB kinase alpha/beta (IKKalpha/beta) phosphorylation and IKKgamma ubiquitination by the ADAP adapter protein. Srivastava R, et al. J Biol Chem, 2010 Apr 9. PMID 20164171.
Phosphorylation of CARMA1 by HPK1 is critical for NF-kappaB activation in T cells. Brenner D, et al. Proc Natl Acad Sci U S A, 2009 Aug 25. PMID 19706536.
COP9 signalosome controls the Carma1-Bcl10-Malt1 complex upon T-cell stimulation. Welteke V, et al. EMBO Rep, 2009 Jun. PMID 19444310.
Compensatory IKKalpha activation of classical NF-kappaB signaling during IKKbeta inhibition identified by an RNA interference sensitization screen. Lam LT, et al. Proc Natl Acad Sci U S A, 2008 Dec 30. PMID 19104039.

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$ 325.00
Cat# AF1190a
(40 western blots)
Availability: 7-10 days
Bulk Size
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