|Application ||WB, E|
|Other Accession||NP_055585, 116986|
|Calculated MW||124674 Da|
|Other Names||Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, AGAP-2, Centaurin-gamma-1, Cnt-g1, GTP-binding and GTPase-activating protein 2, GGAP2, Phosphatidylinositol 3-kinase enhancer, PIKE, AGAP2, CENTG1, KIAA0167|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-CENTG1 / PIKE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.|
|Cellular Location||Isoform 1: Cytoplasm. Nucleus.|
|Tissue Location||Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart|
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Provided below are standard protocols that you may find useful for product applications.
Arf GTPase-activating protein AGAP2 regulates focal adhesion kinase activity and focal adhesion remodeling. Zhu Y, et al. J Biol Chem, 2009 May 15. PMID 19318351.
GGAP2/PIKE-a directly activates both the Akt and nuclear factor-kappaB pathways and promotes prostate cancer progression. Cai Y, et al. Cancer Res, 2009 Feb 1. PMID 19176382.
Cdk5-mediated regulation of the PIKE-A-Akt pathway and glioblastoma cell invasion. Liu R, et al. Proc Natl Acad Sci U S A, 2008 May 27. PMID 18487454.
Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. Tang X, et al. Nat Cell Biol, 2008 Jun. PMID 18469807.
Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase. Liu Z, et al. Mol Cell, 2008 Mar 28. PMID 18374643.
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