|Application ||WB, IHC, E|
|Other Accession||NP_055141, 23604|
|Predicted||Rat, Pig, Dog|
|Calculated MW||42898 Da|
|Other Names||Death-associated protein kinase 2, DAP kinase 2, 220.127.116.11, DAP-kinase-related protein 1, DRP-1, DAPK2|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-DAPK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation.|
|Cellular Location||Cytoplasm. Cytoplasmic vesicle, autophagosome lumen|
|Tissue Location||Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.
Inactivation of RASSF1A, RARbeta2 and DAP-kinase by promoter methylation correlates with lymph node metastasis in nasopharyngeal carcinoma. Fendri A, et al. Cancer Biol Ther, 2009 Mar. PMID 19221469.
Down-regulation of death-associated protein kinase-2 is required for beta-catenin-induced anoikis resistance of malignant epithelial cells. Li H, et al. J Biol Chem, 2009 Jan 23. PMID 18957423.
DAPK2 is a novel E2F1/KLF6 target gene involved in their proapoptotic function. Britschgi A, et al. Oncogene, 2008 Sep 25. PMID 18521079.
The death-associated protein kinase 2 is up-regulated during normal myeloid differentiation and enhances neutrophil maturation in myeloid leukemic cells. Rizzi M, et al. J Leukoc Biol, 2007 Jun. PMID 17347302.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.
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