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Goat Anti-FHL3 / SLIM2 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-FHL3 / SLIM2 Antibody AF1416a
    AF1416a (1 µg/ml) staining of Human Skeletal Muscle lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13643
Other Accession NP_004459, 2275, 56726 (mouse), 302363 (rat)
Reactivity Human, Mouse, Rat
Predicted Pig, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 31192 Da
Additional Information
Gene ID 2275
Other Names Four and a half LIM domains protein 3, FHL-3, Skeletal muscle LIM-protein 2, SLIM-2, FHL3, SLIM2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-FHL3 / SLIM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FHL3
Synonyms SLIM2
Tissue Location Expressed only in skeletal muscle.
Research Areas
Citations (0)

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LIM proteins are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.


Identification of the transactivation domain of the human FHL3. Huang X, et al. Mol Biol (Mosk), 2010 Mar-Apr. PMID 20586194.
Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway. Ding L, et al. J Clin Invest, 2009 Feb. PMID 19139564.
FHL3 binds MyoD and negatively regulates myotube formation. Cottle DL, et al. J Cell Sci, 2007 Apr 15. PMID 17389685.
Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.
The SRF target gene Fhl2 antagonizes RhoA/MAL-dependent activation of SRF. Philippar U, et al. Mol Cell, 2004 Dec 22. PMID 15610731.

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$ 325.00
Cat# AF1416a
(40 western blots)
Availability: 7-10 days
Bulk Size
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