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Goat Anti-GFAP Antibody

Peptide-affinity purified goat antibody

  • IHC - Goat Anti-GFAP Antibody AF1477a
    AF1477a (2 µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • EIA - Goat Anti-GFAP Antibody AF1477a
    AF1477a (5ug/ml) as the reporter with EB002015 as the capture rabbit antibody (5ug/ml). Please note this data was obtained using a previous batch.
  • WB - Goat Anti-GFAP Antibody AF1477a
    AF1477a (0.03 µg/ml) staining of Mouse Brain lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14136
Other Accession NP_002046, 2670, 14580 (mouse), 24387 (rat)
Reactivity Human, Mouse
Predicted Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 49880 Da
Additional Information
Gene ID 2670
Other Names Glial fibrillary acidic protein, GFAP, GFAP
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-GFAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Cellular Location Cytoplasm. Note=Associated with intermediate filaments
Tissue Location Expressed in cells lacking fibronectin.
Research Areas
Citations (0)

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This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.


Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 May 14. PMID 20471030.
Withaferin A targets intermediate filaments glial fibrillary acidic protein and vimentin in a model of retinal gliosis. Bargagna-Mohan P, et al. J Biol Chem, 2010 Mar 5. PMID 20048155.
Specific human astrocyte subtype revealed by affinity purified GFAP antibody; unpurified serum cross-reacts with neurofilament-L in Alzheimer. Middeldorp J, et al. PLoS One, 2009 Nov 4. PMID 19888461.
Redox proteomic analysis of carbonylated brain proteins in mild cognitive impairment and early Alzheimer's disease. Sultana R, et al. Antioxid Redox Signal, 2010 Mar. PMID 19686046.
Glial fibrillary acidic protein in tumor types with cartilaginous differentiation. Santos GC, et al. Mod Pathol, 2009 Oct. PMID 19668151.

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$ 325.00
Cat# AF1477a
(40 western blots)
Availability: 7-10 days
Bulk Size
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