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Goat Anti-P4HA1 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-P4HA1 Antibody AF1772a
    AF1772a (0.2 µg/ml) staining of HEK293 cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13674
Other Accession NP_001017962, 5033, 18451 (mouse), 64475 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 61049 Da
Additional Information
Gene ID 5033
Other Names Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1,, Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-P4HA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name P4HA1
Synonyms P4HA
Function Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Cellular Location Endoplasmic reticulum lumen.
Research Areas
Citations (0)

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This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.


Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Stringency of the 2-His-1-Asp active-site motif in prolyl 4-hydroxylase. Gorres KL, et al. PLoS One, 2009 Nov 5. PMID 19890397.
Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
The length of peptide substrates has a marked effect on hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylases. Koivunen P, et al. J Biol Chem, 2006 Sep 29. PMID 16885164.
Regulation of type II collagen synthesis during osteoarthritis by prolyl-4-hydroxylases: possible influence of low oxygen levels. Grimmer C, et al. Am J Pathol, 2006 Aug. PMID 16877351.

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$ 325.00
Cat# AF1772a
(40 western blots)
Availability: 7-10 days
Bulk Size
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