|Application ||WB, E|
|Other Accession||NP_071919, 64398|
|Calculated MW||77294 Da|
|Other Names||MAGUK p55 subfamily member 5, MPP5|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PALS / MPP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton (By similarity).|
|Cellular Location||Cell membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Cell junction, tight junction. Note=Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1 Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon|
|Tissue Location||Expressed in the retina (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001 [PubMed 11311936]). MPP5 is a member of the p55-like MAGUK subfamily.
Pals1 is a major regulator of the epithelial-like polarization and the extension of the myelin sheath in peripheral nerves. Oz莽elik M, et al. J Neurosci, 2010 Mar 17. PMID 20237282.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
FERM protein EPB41L5 is a novel member of the mammalian CRB-MPP5 polarity complex. Gosens I, et al. Exp Cell Res, 2007 Nov 15. PMID 17920587.
MPP1 links the Usher protein network and the Crumbs protein complex in the retina. Gosens I, et al. Hum Mol Genet, 2007 Aug 15. PMID 17584769.
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