|Application ||WB, E|
|Other Accession||NP_004554, 5106|
|Reactivity||Human, Mouse, Rat, Guinea Pig|
|Predicted||Pig, Dog, Cow|
|Calculated MW||70730 Da|
|Other Names||Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, 220.127.116.11, PCK2, PEPCK2|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PCK2 / PEPCK-M Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
COMMON VARIANTS IN 40 GENES ASSESSED FOR DIABETES INCIDENCE AND RESPONSE TO METFORMIN AND LIFESTYLE INTERVENTIONS IN THE DIABETES PREVENTION PROGRAM. Jablonski KA, et al. Diabetes, 2010 Aug 3. PMID 20682687.
Fasting hyperglycemia is not associated with increased expression of PEPCK or G6Pc in patients with Type 2 Diabetes. Samuel VT, et al. Proc Natl Acad Sci U S A, 2009 Jul 21. PMID 19587243.
Endurance capacity, not body size, determines physical activity levels: role of skeletal muscle PEPCK. Novak CM, et al. PLoS One, 2009 Jun 12. PMID 19521512.
Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations. Lu Y, et al. J Lipid Res, 2008 Dec. PMID 18660489.
AMP-activated protein kinase regulates PEPCK gene expression by direct phosphorylation of a novel zinc finger transcription factor. Inoue E, et al. Biochem Biophys Res Commun, 2006 Dec 29. PMID 17097062.
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