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Goat Anti-PPP2R5A Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-PPP2R5A Antibody AF1856a
    AF1856a staining (0.5 µg/ml) of human muscle lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • IHC - Goat Anti-PPP2R5A Antibody AF1856a
    AF1856a (5 µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15172
Other Accession NP_006234, 5525
Reactivity Human
Predicted Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 56194 Da
Additional Information
Gene ID 5525
Other Names Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform, PP2A B subunit isoform B'-alpha, PP2A B subunit isoform B56-alpha, PP2A B subunit isoform PR61-alpha, PR61alpha, PP2A B subunit isoform R5-alpha, PPP2R5A
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-PPP2R5A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP2R5A
Function The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Cellular Location Cytoplasm. Nucleus. Chromosome, centromere. Note=From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase.
Tissue Location Widely expressed with the highest expression in heart and skeletal muscle
Research Areas
Citations (0)

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The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily.


Nuclear export and centrosome targeting of the protein phosphatase 2A subunit B56alpha: role of B56alpha in nuclear export of the catalytic subunit. Flegg CP, et al. J Biol Chem, 2010 Jun 11. PMID 20378546.
Negative regulation of CHK2 activity by protein phosphatase 2A is modulated by DNA damage. Freeman AK, et al. Cell Cycle, 2010 Feb 15. PMID 20160490.
The Balpha and Bdelta regulatory subunits of PP2A are necessary for assembly of the CaMKIV.PP2A signaling complex. Reece KM, et al. Biochem Biophys Res Commun, 2009 Sep 4. PMID 19538941.
Lysine-independent turnover of cyclin G1 can be stabilized by B'alpha subunits of protein phosphatase 2A. Li H, et al. Mol Cell Biol, 2009 Feb. PMID 18981217.
PKR regulates B56(alpha)-mediated BCL2 phosphatase activity in acute lymphoblastic leukemia-derived REH cells. Ruvolo VR, et al. J Biol Chem, 2008 Dec 19. PMID 18957415.

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$ 325.00
Cat# AF1856a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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